Hamid Liryaei, Hooman Kaghazian, Mohammad Reza Akbari Eidgahi

Abstract

Ultrafiltration plays an important role in purification process of recombinant proteins. In this study, the efficiency of the process was assessed by changing buffering condition. Initially, the harvests were purified by primary chromatographic methods. Then, the samples were applied to the ultrafiltration and washed with different pH and conductivity buffers. Proteins purity tests were done and the peptide map investigated by the RP-HPLC method. The research results showed that changes in buffering conditions can improve the efficacy of ultrafiltration and some physicochemical properties had a significant effects on the final resultsUltrafiltration plays an important role in purification process of recombinant proteins. In this study, the efficiency of the process was assessed by changing buffering condition. Initially, the harvests were purified by primary chromatographic methods. Then, the samples were applied to the ultrafiltration and washed with different pH and conductivity buffers. Proteins purity tests were done and the peptide map investigated by the RP-HPLC method. The research results showed that changes in buffering conditions can improve the efficacy of ultrafiltration and some physicochemical properties had a significant effects on the final results.