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Isocratic Reverse Phase High Performance Liquid Chromatographic Estimation of Ramipril and Amlodipine in Pharmaceutical Dosage Form

Manikanta Kumar. A , P. Vijay Kumar* , Mahesh Nasare , Venkateswar Rao , V.V.L.N. Prasad , Prakash V. Diwan


A new simple, rapid, specific, accurate, precise and novel reverse phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the simultaneous estimation of Ramipril and amlodipine besylate in the combined pharmaceutical dosage form. The chromatographic separation for ramipril and amlodipine besylate were achieved with mobile phase containing mixed phosphate buffer (0.02 M potassium di-hydrogen orthophosphate and 0.002 M di-potassium hydrogen phosphate anhydrous, pH 6.8) and acetonitrile (60:40 % v/v), phenomenex C18 column (150 × 4.6mm i.d, particle size of 5μ) at 5⁰ C and UV detection at 237 nm. The compounds were eluted in the isocratic mode at a flow rate of 1 ml min-1. The retention times of Ramipril 2.13 ± 0.09 min and amlodipine besylate at 5.18 ± 0.12 min. The above method was validated in terms of linearity, accuracy, precision, LOD, LOQ etc. in accordance with ICH guidelines.

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