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Development and validation of a HPLC analytical assay method for amlodipine besylate tablets: A Potent Ca+2 channel blocker


Richa Sah and Saahil Arora

Abstract

Amlodipine besylate is a potent calcium channel blocker used for the treatment of hypertension, congestive heart failure and angina pectoris. Amlodipine besylate avoids the adverse effect of amlodipine in racemic mixtures. A highly precise and cost effective RP-HPLC method with retention time of 2.60 minutes was developed for the estimation of amlodipine besylate in tablet dosage form, by fixing the parameters as WATERS C18 column 250 mm × 4.6 mm (5μm), with mobile phase as acetonitrile: 70mM potassium dihydrogen orthophosphate buffer: methanol (15:30:55) and pH adjusted to 3.0 using OPA. Mobile phase flow rate was maintained at 1.0 ml/min and detected at 240nm.



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