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Screening of in-vitroAntioxidant Profile of Different Extracts of the Leaves of Plumeria alba Linn


Samyak Chaudhuri , Sudeshna Bakshi , J. N. Pande , Moulisha Biswas

Abstract

Production of reactive oxygen species (ROS) causes various diseases and cellular anomalies in human beings. Antioxidants inhibit generation of reactive species by scavenging them. Plumeria alba Linn commonly known as White Champa (family: Apocynaceae). It is used to treat ulcers, herpes, scabies and seeds possess haemostatic properties. Till date no antioxidant activity had been reported on the leaves of Plumeria alba; therefore, my project work has been conceptualized on the free radical scavenging activity of the leaves of Plumeria alba. The present study assessed the phytochemical analysis mainly successive extractive values, thin layer chromatographic profile and phytochemical screening of petroleum ether, chloroform and methanol extracts of Plumeria alba leaves. The in vitro antioxidant potential by DPPH radical scavenging assay was also estimated for each of the extracts with the reference drug Ascorbic acid. Total chromatographic profile was obtained for each extracts. Presence of phytoconstituents such as flavonoids, polyphenols, terpenoids and glycoside in different extracts were found. The potent free radical scavenging activity was observed on DPPH radical scavenging assay. The different concentrations of all the extracts showed effective free radical scavenging activity on dose dependant manner.



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