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Nigella sativa protects Kidneys against Metabolic disorders induced by high fat diet in Rats


Rasha Alshali , Aziza Alrafiah , Ebtisam Al-ofi and et al

Abstract

Introduction: High fat diet (HFD) intake initiate variety of metabolic disorders as insulin resistance and type 2 diabetes. Adipose tissue secretes adiopocytokines, which cause lipid peroxidation in cell membrane of vital organs like kidneys. Nigella sativa (NS) is a famous condiment; it is a medicinal herb, which contains number of active contents and has no toxic effects. Methods: We applied 8 weeks HFD feeding protocol to induce metabolic disorders in rats which were divided randomly into three groups; control group, HFD group and NS group. Kidney function parameters (urea, uric acid and creatinine), Lipid peroxidation marker (Malondialdehyde MDA) and oxidative stress biomarkers (glutathione GSH – superoxide dismutase SOD) were measured. Paraffin sections were stained with different stains to examine histological architecture and collagen contents in renal tissues. Results: HFD feeding protocol resulted in disturbances in kidney function tests; there were rise in urea, uric acid and creatinine values; increased values of lipid peroxidation marker MDA and decreased values of oxidative stress markers GSH and SOD were observed. Histologically, there was batches of affected renal areas showing glomerulomegally, shortened epithelium of proximal convoluted tubules, dilatation of blood vessels (glomerular capillaries and rete mirabile). In NS group, kidney function tests were normal and plasma levels of biochemical markers MDA, GSH and SOD were around its normal values. NS preserved normal renal architecture. Conclusion: Administration of Nigella sativa with HFD ameliorated the adverse effects of HFD on kidneys through a mechanism utilizing NS antioxidant activity.




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