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Evaluation of safety and in-Vitro Efficacy study of Anti-Fungal Cream


Kruti N Pandya , Alok J Shah , Sonal S Patel , Vidhi B Parikh , Ghanshyam R Patel , Hardik K Soni

Abstract

Evaluation of Antifungal activity of herbo-mineral formulation and its ingredients were tested In-vitro against yeast Candida albicans by various methods using Miconazole as standard drug. Simultaneously safety study (skin irritation test) of herbal-mineral formulation was also performed according to OECD guidelines. Safety of the formulation was proved by skin irritation test and efficacy of the formulation was proved by In-vitro antifungal bio-assay. Materials & Methods: The product was first checked for its solubility followed by anti fungal activity using both the Cup Method and Kirby Bauer Method. The Skin irritation test was carried out on Healthy adult male Wistar rats weighing 250-300g. All experiments and protocols described in present study were approved by the Institutional Animal Ethics Committee (IAEC) (Approval No.: KBIPER/IAEC/2012/353) of KBIPER (K.B. Institute of pharmaceutical education and research) and with permission from Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) (Reg. No.: 35/1999/CPCSEA), Ministry of Social Justice and Empowerment, Government of India Results & Discussion: The study clearly shows that the herbo-mineral formulation is showing good in-vitro anti-fungal activity against C. albicans and no skin irritation or adverse effects were observed in animals during the entire study.



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