TY  - JOUR
T1  - Hepatoprotective effect of Olea europaea L. seeds extracts against methotrexate induced liver injury in mice
A1  - Nada Sahib Shaker
A1  - Zeena Ayad Hussein
A1  - Nibras Jamal Tahseen
A1  - Amjed Shawqy Al-Musalahi
A1  - Hayder Bahaa Sahib
JF  - Journal of Advanced Pharmacy Education and Research
JO  - J Adv Pharm Educ Res
SN  - 2249-3379
Y1  - 2022
VL  - 12
IS  - 3
DO  - 10.51847/FERn3EhDzZ
SP  - 113
EP  - 121
N2  - The present study aimed to determine the hepatoprotective effect of different extracts of Olea europaea L. seeds in mice induced with MTX-induced liver injury and find out the most effective extract. Olea europaea L. (olive) seeds powder was extracted with three solvents with different polarities in a sequential manner (chloroform, methanol, and 50% aqueous ethanol) using the cold method. 48 male albino mice were divided into six groups. Mice were pretreated orally with silymarin (standard) and the test extracts for 5 consecutive days at a dose of 200mg/kg and 500mg/kg consecutively. Mice received methotrexate (20mg/kg) IP single dose on day 5. On day 6, blood samples were collected for biochemical analysis of serum liver indices; then animals were sacrificed to collect liver tissues for histological examination. The antioxidant activity for each extract was assessed using the DPPH assay. The results indicated that MTX successfully induced liver damage that was observed through marked elevation of serum GPT, GOT, and ALP as well as alterations in the cellular structure of liver tissue. All extracts of olive seeds were able to reduce serum levels of liver indices, although 50% ethanol extract was revealed to be the best in reducing levels of GPT, GOT, and ALP and preserve liver tissue cellular structure, as well as having the highest free radical scavenging activity in DPPH assay. The study suggests that 50% ethanol extract of Olea europaea L. seeds can be safely used with MTX therapy to reduce its hepatic toxic effect‎.
UR  - https://japer.in/article/hepatoprotective-effect-of-olea-europaea-l-seeds-extracts-against-methotrexate-induced-liver-injury-a5c8rwaelcf8zfv
ER  -