TY  - JOUR
T1  - Attenuation of alginate lyase by chlorhexidine in the alginate pathway of Pseudomonas aeruginosa biofilm formation
A1  - Mohammad Subkhan
A1  - Sukardiman Sukardiman
A1  - Isnin Anang Marhana
A1  - Laily Irfana
JF  - Journal of Advanced Pharmacy Education and Research
JO  - J Adv Pharm Educ Res
SN  - 2249-3379
Y1  - 2025
VL  - 15
IS  - 1
DO  - 10.51847/lYQJ0dBLMr
SP  - 43
EP  - 48
N2  - Ventilator-associated pneumonia (VAP) caused by Pseudomonas aeruginosa poses a significant clinical challenge due to the pathogen's robust biofilm formation and resistance mechanisms. Chlorhexidine (CHX), a commonly used antiseptic, has shown potential in biofilm inhibition, though its exact mechanism remains unclear. Our previous work focusing on quorum sensing associated proteins identified strong CHX binding to PslG, a key protein in the Psl pathway. However, the potential for CHX interactions within other biofilm pathways, such as alginate, has not been explored. This study explored CHX’s potential interaction with proteins involved in the alginate pathway of P. aeruginosa biofilm formation using reverse docking. Structures of eight alginate-associated proteins were retrieved from the Protein Data Bank. The CHX structure was obtained from PubChem and docking simulations were conducted using Fpocket and SMINA to predict binding affinities. A total of 735 docking poses were generated for the analyzed alginate-associated proteins. Among these, CHX showed the strongest binding affinity for alginate lyase, a key enzyme that degrades alginate and has been implicated as a therapeutic target for biofilm disruption. This finding suggests that CHX may exert its anti-biofilm effects by targeting the alginate matrix through alginate lyase, potentially enhancing biofilm degradation. Given the promising application of alginate lyase in biofilm control strategies, the interaction between CHX and alginate lyase merits further experimental validation to explore its therapeutic impact in clinical settings‎.
UR  - https://japer.in/article/attenuation-of-alginate-lyase-by-chlorhexidine-in-the-alginate-pathway-of-pseudomonas-aeruginosa-bio-gesdtiaiwjvpnzm
ER  -