%0 Journal Article
%T Anticancer, cytotoxicity, and genotoxicity assays of Tulbaghia violacea extracts
%A Dijeng Euginiah Rampana
%A Pakiso Moses Makhoahle
%A Sitheni Samson Mashele
%J Journal of Advanced Pharmacy Education and Research
%@ 2249-3379
%D 2022
%V 12
%N 4
%R 10.51847/Ceo7ssgJdO
%P 27-31
%X It has been shown that herbal medicine is a rich source of treatments with several chemical structures and bioactive elements that are effective against a variety of illnesses. Using the Sulforhodomine B(SBR) assay, T. violacea was examined for anticancer activity in this study to ascertain the growth-inhibiting properties of the water and methanol/dichloromethane plant extracts. Thermo Fischer Scientific product, the NucRed Live 647 Probe was used in the genotoxicity experiment to measure the growth of micronuclei. The SRB test was performed to evaluate the cytotoxic activity on the WI38 cell line. When examined with water and methanol/dichloromethane extracts, PC3 (prostate), HeLa (cervix), and the human cell lines TK-10 (renal) showed no activity. No genotoxicity was evident in the extracts made from water or methanol/dichloromethane. When tested against, neither the water extract nor the methanol/dichloromethane extract of the WI-38 cell line. Aqueous proved to be an effective solvent for T. violacea plant extraction. The plant's leaf extracts were recommended as the best extract to test on the PC-3, HeLa human cell lines, and TK-10. This study, therefore, agrees with the traditional methods of extraction and the preferred plant part used by traditional healers‎.
%U https://japer.in/article/anticancer-cytotoxicity-and-genotoxicity-assays-of-tulbaghia-violacea-extracts-almyoh2ig5agli1