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Review Article
Year :  |  Volume : 7   |  Issue :1   |  Page :28-30  


Priyangaa Sathasivam, T. Lakshmi

Correspondence Address:Department of Pharmacology, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu, India

Source of Support: None , Conflict of Interest: None


DOI: 10.4103/2231-4040.197331

  Abstract  
How to cite this article:

Sathasivam P, Lakshmi T. Brine shrimp lethality assay of Sesbania grandiflora ethanolic extract – in vitro study. J Adv Pharm Edu Res 2017;7(1):28-30.


  Introduction   Top

Sesbania grandiflora is commonly known as Agathi keerai in Tamil. It is a popularly used herbal extract. Various literatures prove that there are about 60 global species belonging to genus Sesbania which are commonly found to be grown in Africa, Australia, and Asia. S. grandiflora is commonly found in tropical regions. The vernacular name of S. grandiflora is also known as agusta, bagphal, and agate (Bengali).[1,2] S. grandiflora is a small, erect, fast growing perennial (4-5 m in just 6 months), and sparsely branched tree that reaches 10-15 m in height and diameter up to 12 cm.[3] It is traditionally used for anti-inflammation,[4] antimicrobial activities,[5,6] anticancer,[7] antioxidative activities,[8,9] anti-ulcer activity,[10,11] and immunomodulatory activity,[12] and associated diseases such as hepatic diseases,[13,14] respiratory diseases,[15] and renal diseases.[16] The juice of the bark or root is traditionally used for the treatment of cancer in India.[17] Brine shrimp lethality bioassay is a simple, high throughput cytotoxicity test of bioactive chemicals. It is based on killing the ability of test compounds on a simple zoological organismbrine shrimp (Artemia salina). [18] Brine shrimp lethality bioassay is widely used in the evaluation of toxicity of heavy metals, pesticides, medicines especially natural plant extracts, etc.[19,20] Hence with this regard, we have chosen S. grandiflora leaf against the brine shrimp to evaluate the lethality assay. 

  Materials and methods   Top

Plant material

S. grandiflora leaf was obtained as a gift sample from Green Chem Lab, Bengaluru, India.

Brine shrimp lethality assay

The eggs of brine shrimp were procured from Philadelphia, USA. In a small water tank containing seawater, the eggs were incubated for 48 h for hatching. Required light was provided with Philips 40 Watts lamp for 12 h cycle. After 24 h, the larvae were used for the experiments. The nauplii of brine shrimp were challenged in different test tubes containing 10 mL of sea water and 20 larvae. To this, extracts of leaves at different concentrations (10-50 mg/mL) were added. After 24 h, the viability of larvae was observed, and mortality was recorded. Nauplii were considered dead when they were immobile and stayed at the bottom of the test tubes.[21-23] The percent mortality of brine shrimp was calculated with the formula given below.

[% mortality = Number of brine shrimp dead/Number of brine shrimp introduced ×100]

  Results   Top

Discussion   Top

Conclusion   Top

References   Top

Figures

 

 

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