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Original Article
Year :  |  Volume : 7   |  Issue :1   |  Page :22-24  


Ilakkiya Ezhil, T. Lakshmi

Correspondence Address:Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha University, Chennai, Tamil Nadu, India

Source of Support: None , Conflict of Interest: None


DOI: 10.4103/2231-4040.197331

  Abstract  
How to cite this article:

Ezhil I, Lakshmi T. Antibacterial efficacy of Epicatechin and Rutin from Acacia catechu leaf extract against Enterococcus faecalis and Streptococcus mutans -An in vitro study. J Adv Pharm Edu Res 2017;7(1):22-24.


  Introduction   Top

Oral microbial flora is dominated by gram positive micro organisms and hence dental plaque which is formed on the tooth surface contains gram positive cocci and bacilli.[1] Since decades Natural products have been investigated as promising agents to prevent oral diseases like Dental caries,Dental plaque, periodontal disease.[2]

Acacia catechu Willd. (Mimosaceae) is commonly called as khair or black cutch. It is known as khadira in Sanskrit and Karungali in Tamil.[3] People in Kerala drink the boiled juice for digestive disorders. In India.[4] Epicatechin is an odorless white powder. Epcatechin is a flavonol belonging to the group of flavonoids. Epicatechin is a strong antioxidant, has insulin mimic action and improves heart health.It is used in management of diabetes mellitus.[5-7] Epicatechin 3 gallate is the most predominant catechins which is used to treat dental caries and periodontal infections.[8] Rutin is a bioflavonoid. Pure rutin is yellow or yellow-green coloured needle-shaped crystal. Rutin is a flavonol glycoside comprised of the quercetin and the disaccharide rutinose. The richest source is buckwheat. Rutin is also found in citrus fruits, noni, black tea, apple peel. During digestion much of the rutin is metabolized to its aglycone, quercetin.[9] It has antioxidant ,anti inflammatory ,anticancer and antiobesity activity.[10-12] Hence, in this study, we investigated the antibacterial activity of Epicatechin and Rutin from Acacia catechu leaf extract against oral bacteria.

  Materials and methods   Top

Drugs

Epicatechin and rutin were purchased from Sigma-Aldrich, India.

Test microorganism

Streptococcus mutans -ATCC 25175 Enterococcus faecalis -ATCC 29212.

Minimum inhibitory concentration (MIC)[13-15]

To 100 µL of sterile broth, 100 µL of the sample was added and mixed to attain a concentration of 10 mg/mL. This was serially diluted to obtain the dilutions of 50 mg/mL, 25 mg/mL, 15 mg/mL, 10 mg/mL, 5 mg/mL, 2.5 mg/mL, and finally 1 mg/mL. Each concentration was inoculated with 0.01 mL of 24 h bacterial cell suspension and incubated at 37°C for 24 h. Chlorhexidine is used as a positive control. The presence of cloudiness or turbidity of the broth indicates positive growth. The concentration which inhibits the bacterial growth is considered as the MIC.

  Results   Top

Discussion   Top

Conclusion   Top

References   Top

Figures

 

 

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